![]() ![]() EtBr binds to the DNA and allows you to visualize the DNA under ultraviolet (UV) light.ĬAUTION: EtBr is a known mutagen. (Optional) Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). ![]() Let agarose solution cool down to about 50 ☌ (about when you can comfortably keep your hand on the flask), about 5 mins. Placing saran wrap over the top of the flask can help with this, but is not necessary if you pay close attention. Keep an eye on it the solution has a tendancy to boil over. *Pro-Tip* It is a good idea to microwave for 30-45 sec, stop and swirl, and then continue towards a boil.
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